Physicochemical Properties
| Molecular Formula | C21H16F4N8O |
| Molecular Weight | 472.40 |
| Appearance | Typically exists as solid at room temperature |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | JH-XVII-10 (compound 10) (1 µM; CAL27 cells) exhibits activity against the following: FAK (IC50=90 nM), RSK1 (IC50=82 nM), RSK2 (IC50=80 nM), and RSK3 (IC50=61 nM). For CAL27 and FaDu cells, JH-XVII-10 (10 µM; 72 h) reduces cell growth by approximately 45% and 40%, respectively[1]. In CAL27 cells, JH-XVII-10 (1, 10 µM; 24 h) promotes apoptosis[1]. CAL27 cells exposed to JH-XVII-10 (0.5, 1, 5, 10 µM; 24 h) exhibit pro-tumor signaling inhibition[1]. |
| ln Vivo | The oral bioavailability (F=12%) of JH-XVII-10 (2 mg/kg, iv; 10 mg/kg, po) is reported[1]. JH-XVII-10 pharmacokinetic parameters in male C57Bl/6 mice[1]. Administration parameters: rat dog iv T1/2 (h) 1.4±0.3 5.70±1.2 AUC0-∞ (ng*h/mL) 931.3±95.7 14,830.8±5475.4 CL (mL/min/kg) 17.6±2.0 149.9±62.5 Vss (L/kg) 1.7±0.2 828.7±134.2 po Cmax (ng/mL) 1661.1±916.6 3979.4±483.5 Tmax(h) 0.9±0.8 1.3±0.5 T1/2 (h) 1.4±0.2 4.9±0.6 AUC0-∞ (ng *h/mL) 5044.9±1061 23,109.9±7752.2 F (%) 54.2 31.8 C57Bl/6 male mice; 2 mg/kg, iv; 10 mg/kg, po[1]. |
| Cell Assay |
Cell Proliferation Assay[1] Cell Types: CAL27, FaDu, HEK293FT cells Tested Concentrations: 10 µM Incubation Duration: 72 h Experimental Results: diminished cell proliferation by ~45%, and ~40% for CAL27 and FaDu cells, respectively. Western Blot Analysis[1] Cell Types: CAL27 cells Tested Concentrations: 0.5, 1, 5, 10 µM Incubation Duration: 24 h Experimental Results: demonstrated inhibitory effects on pro -tumor signaling. Apoptosis Analysis[1] Cell Types: CAL27 cells Tested Concentrations: 1, 10 µM Incubation Duration: 24 h Experimental Results: Induced increases in the proapoptotic marker (cleaved PARP), and diminished the expression of antiapoptotic protein BCL-xL. |
| Animal Protocol |
Animal/Disease Models: C57Bl/6 male mice[1] Doses: Route of Administration: 2 mg/kg, iv; 10 mg/kg, po Experimental Results: demonstrated oral bioavailability (F=12%). |
| References |
[1]. Selective Macrocyclic Inhibitors of DYRK1A/B. ACS Med Chem Lett. 2022; 13(4):577-585. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1169 mL | 10.5843 mL | 21.1685 mL | |
| 5 mM | 0.4234 mL | 2.1169 mL | 4.2337 mL | |
| 10 mM | 0.2117 mL | 1.0584 mL | 2.1169 mL |