JC124 is a specific NLRP3 inflammasome inhibitor. JC124 has anti~inflammatory and neuro-protective (neuro-protection) effects.

Physicochemical Properties

Molecular Formula	C17H19CLN2O4S
Molecular Weight	382.861762285233
Exact Mass	382.075
Elemental Analysis	C, 53.33; H, 5.00; Cl, 9.26; N, 7.32; O, 16.72; S, 8.37
CAS #	1638611-48-9
Related CAS #	1638611-48-9;
PubChem CID	117715670
Appearance	Light yellow to yellow solid powder
LogP	2.8
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	7
Heavy Atom Count	25
Complexity	529
Defined Atom Stereocenter Count	0
InChi Key	LXYPQRJNQPYTQW-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H19ClN2O4S/c1-19-25(22,23)14-6-3-12(4-7-14)9-10-20-17(21)15-11-13(18)5-8-16(15)24-2/h3-8,11,19H,9-10H2,1-2H3,(H,20,21)
Chemical Name	5-chloro-2-methoxy-N-[2-[4-(methylsulfamoyl)phenyl]ethyl]benzamide
Synonyms	JC 124; JC-124; JC124
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	It has been demonstrated that JC124 can specifically prevent the production of NLRP3 inflammasomes, activate caspase-1, and lower IL-1β in vitro[1].
ln Vivo	Injury-induced deteriorated neuronal numbers, inflammatory cell responses in injured brain, and cortical lesion volume were all significantly reduced by JC124 (100 mg/kg; i.p.; 4 doses; 30 minutes post-injury, 6, 24, and 30 hours post-TBI) treatment. The protein expression levels of NLRP3, ASC, IL-1 beta, TNFα, iNOS, and caspase-1 are similarly markedly decreased by JC124 [1].
Animal Protocol	Animal/Disease Models: Adult male SD (SD (Sprague-Dawley)) rats (3-4 months old) with traumatic brain injury (TBI) [1] Doses: 100 mg/kg Route of Administration: intraperitoneal (ip) injection; 4 doses; 30 days after injury Minutes, 6, 24, and 30 hrs (hrs (hours)) after TBI Experimental Results: Significant reduction in the number of injury-induced degenerated neurons, inflammatory cell responses in the injured brain, and cortical lesion volume.
References	[1]. A novel small molecular NLRP3 inflammasome inhibitor alleviates neuroinflammatory response following traumatic brain injury. J Neuroinflammation. 2019 Apr 11;16(1):81.

Solubility Data

Solubility (In Vitro)

DMSO : ~250 mg/mL (~652.98 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (5.43 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (5.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6119 mL	13.0596 mL	26.1192 mL
	5 mM	0.5224 mL	2.6119 mL	5.2238 mL
	10 mM	0.2612 mL	1.3060 mL	2.6119 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.