JB061 is a non-muscle myosin inhibitor (antagonist) with IC50s of 4.4 μM (cardiac myosin), 9.1 μM (skeletal muscle myosin), and >100 μM (smooth muscle myosin II). JB061 has a low ability to inhibit ATPase activity (IC50>200 μM). JB061 has a cytotoxic effect on COS-7 cells with IC50 of 39 μM.

Physicochemical Properties

Molecular Formula	C19H17NO4
Molecular Weight	323.34
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Cardiac muscle myosin 4.4 μM (IC50) Skeletal muscle myosin 9.1 μM (IC50) Smooth muscle myosin II >100 μM (IC50)
ln Vitro	In Cos-7 cells, JB061 (compound 6a) (40 μM; 24 h) suppresses cytokinesis [1].
References	[1]. Isoform selectivities of novel 4-hydroxycoumarin imines as inhibitors of myosin II. Eur J Med Chem. 2022 Dec 12;247:115008.

Solubility Data

Solubility (In Vitro)

DMSO :~25 mg/mL (~77.32 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (6.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0927 mL	15.4636 mL	30.9272 mL
	5 mM	0.6185 mL	3.0927 mL	6.1854 mL
	10 mM	0.3093 mL	1.5464 mL	3.0927 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.