J30-8 is a highly efficient, isoform-selective JNK3 conjugate with IC50 of 40 nM and 2500-fold selectivity over JNK1α1 and JNK2α2 isoforms. J30-8 has neuro-protection activity in vitro and has current potential for studying neurodegeneration.

Physicochemical Properties

Molecular Formula	C17H9CLFN3O2S
Molecular Weight	373.788664579391
Exact Mass	373.008
CAS #	2366255-71-0
PubChem CID	4078145
Appearance	Brown to red solid powder
LogP	3.9
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	3
Heavy Atom Count	25
Complexity	721
Defined Atom Stereocenter Count	0
SMILES	ClC1C=CC=CC=1NC1=NC(=C(C2C(N=C3C=CC(=CC=23)F)=O)S1)O
InChi Key	BDCJVPSBCYWSSS-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H9ClFN3O2S/c18-10-3-1-2-4-12(10)21-17-22-16(24)14(25-17)13-9-7-8(19)5-6-11(9)20-15(13)23/h1-7,24H,(H,21,22)
Chemical Name	3-[2-(2-chloroanilino)-4-hydroxy-1,3-thiazol-5-yl]-5-fluoroindol-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Multistage Screening Reveals 3-Substituted Indolin-2-one Derivatives as Novel and Isoform-Selective c-Jun N-terminal Kinase 3 (JNK3) Inhibitors: Implications to Drug Discovery for Potential Treatment of Neurodegenerative Diseases. J Med Chem. 2019 Jul 25;62(14):6645-6664.

Solubility Data

Solubility (In Vitro)

DMSO : ~6.67 mg/mL (~17.84 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 0.67 mg/mL (1.79 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6753 mL	13.3765 mL	26.7530 mL
	5 mM	0.5351 mL	2.6753 mL	5.3506 mL
	10 mM	0.2675 mL	1.3376 mL	2.6753 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.