Isepamicin sulfate (Sch 21420 sulfate) is a broad-spectrum aminoglycoside antibiotic. Isepamicin sulfate has considerable anti-bacterial effect against some Gram-negative (Gram+) non-fermenting bacteria with high antimicrobial resistance.

Physicochemical Properties

Molecular Formula	C22H43N5O12.XH2SO4
Molecular Weight	665.6657
Exact Mass	665.243
CAS #	67814-76-0
Related CAS #	Isepamicin;58152-03-7
PubChem CID	171883
Appearance	White to off-white solid powder
Density	1.53g/cm3
Boiling Point	926.8ºC at 760mmHg
Flash Point	514.3ºC
Hydrogen Bond Donor Count	12
Hydrogen Bond Acceptor Count	20
Rotatable Bond Count	9
Heavy Atom Count	44
Complexity	880
Defined Atom Stereocenter Count	15
SMILES	S(=O)(=O)([O-])[O-].O([C@]1([H])[C@@]([H])([C@]([H])([C@](C([H])([H])[H])(C([H])([H])O1)O[H])N([H])C([H])([H])[H])O[H])[C@]1([H])[C@]([H])([C@@]([H])([C@]([H])(C([H])([H])[C@@]1([H])N([H])C([C@]([H])(C([H])([H])N([H])[H])O[H])=O)N([H])[H])O[C@]1([H])[C@@]([H])([C@]([H])([C@@]([H])([C@@]([H])(C([H])([H])N([H])[H])O1)O[H])O[H])O[H])O[H]
InChi Key	DDXRHRXGIWOVDQ-MGAUJLSLSA-L
InChi Code	InChI=1S/C22H43N5O12.H2O4S/c1-22(35)6-36-20(15(33)18(22)26-2)39-17-8(27-19(34)9(28)4-23)3-7(25)16(14(17)32)38-21-13(31)12(30)11(29)10(5-24)37-21;1-5(2,3)4/h7-18,20-21,26,28-33,35H,3-6,23-25H2,1-2H3,(H,27,34);(H2,1,2,3,4)/p-2/t7-,8+,9-,10+,11+,12-,13+,14-,15+,16+,17-,18+,20+,21+,22-;/m0./s1
Chemical Name	(2S)-3-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-2-[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy-3-hydroxycyclohexyl]-2-hydroxypropanamide;sulfate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Aminoglycoside
References	[1]. Samonis G, et al. Antimicrobial susceptibility of non-fermenting Gram-negative isolates to isepamicin in a region with high antibiotic resistance. Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):3191-8.
Additional Infomation	See also: Isepamicin Sulfate (annotation moved to).

Solubility Data

Solubility (In Vitro)	H2O : 125 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5022 mL	7.5112 mL	15.0225 mL
	5 mM	0.3004 mL	1.5022 mL	3.0045 mL
	10 mM	0.1502 mL	0.7511 mL	1.5022 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.