Imidocarb dipropionate is an antiprotozoal agent with activity against the parasite B. bovis (IC50 of 87 μg/mL). It eliminates blood parasites and has been used in the veterinary treatment of babesiosis and other protozoal infections.

Physicochemical Properties

Molecular Formula	C25H32N6O5
Molecular Weight	496.5588
Exact Mass	496.243
Elemental Analysis	C, 60.47; H, 6.50; N, 16.92; O, 16.11
CAS #	55750-06-6
Related CAS #	5318-76-3 (HCl);55750-06-6 (propionate);27885-92-3;
PubChem CID	9983292
Appearance	Solid powder
Density	1.39g/cm3
Melting Point	205ºC
LogP	2.667
Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	6
Heavy Atom Count	36
Complexity	567
Defined Atom Stereocenter Count	0
SMILES	O=C(N([H])C1=C([H])C([H])=C([H])C(=C1[H])C1=NC([H])([H])C([H])([H])N1[H])N([H])C1=C([H])C([H])=C([H])C(=C1[H])C1=NC([H])([H])C([H])([H])N1[H].O([H])C(C([H])([H])C([H])([H])[H])=O.O([H])C(C([H])([H])C([H])([H])[H])=O
InChi Key	AFGQXWSHYUHHNV-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H20N6O.2C3H6O2/c26-19(24-15-5-1-3-13(11-15)17-20-7-8-21-17)25-16-6-2-4-14(12-16)18-22-9-10-23-18;2*1-2-3(4)5/h1-6,11-12H,7-10H2,(H,20,21)(H,22,23)(H2,24,25,26);2*2H2,1H3,(H,4,5)
Chemical Name	N,N'-bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea, dipropanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Parasite B. bovis(IC50= 87 μg/mL)
ln Vivo	Horses receive injections of imidocarb dipropionate subcutaneously or intramuscularly (3.4 mg/kg bw) and cattle (2.1 mg/kg bw). Horses may receive up to four doses spaced seventy-two hours apart. After two weeks, a second dose may be administered[1]. In sheep, imidocarb dipropionate (1. 2 mg/kg bw) is also used to treat anaplasmosis and babesiosis[1]. Imidocarb dipropionate's acute oral LD50 in mice ranges from 646 to 723 mg/kg bw[1]. In rats, imidocarb's acute oral LD50 ranges from 454 to 1251 mg/kg bw[1].
References	[1]. In vitro responsiveness of Babesia bovis to Imidocarb dipropionate and the selection of a drug-adapted line. Vet Parasitol. 1996 Mar;62(1-2):35-41.
Additional Infomation	See also: Imidocarb (has active moiety).

Solubility Data

Solubility (In Vitro)

DMSO : ~10.42 mg/mL (~20.98 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.04 mg/mL (2.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.4 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.04 mg/mL (2.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.4 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.04 mg/mL (2.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.4 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0139 mL	10.0693 mL	20.1386 mL
	5 mM	0.4028 mL	2.0139 mL	4.0277 mL
	10 mM	0.2014 mL	1.0069 mL	2.0139 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.