IMB-301 is a specific HIV-1 replication inhibitor that can bind to hA3G (human APOBEC3G), block hA3G-Vif interaction, and inhibit Vif-mediated hA3G degradation. IMB-301 inhibits HIV-1 replication in H9 cells (IC50=8.63 uM). Human APOBEC3G is a restriction factor that can inhibit HIV-1 replication.

Physicochemical Properties

Molecular Formula	C19H17CL2FN2O
Molecular Weight	379.26
Exact Mass	378.07
CAS #	64009-84-3
PubChem CID	327197
Appearance	Colorless to light yellow oil
Density	1.27g/cm3
Boiling Point	548.1ºC at 760mmHg
Flash Point	285.3ºC
Index of Refraction	1.589
LogP	5.581
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	7
Heavy Atom Count	25
Complexity	396
Defined Atom Stereocenter Count	0
SMILES	FC1=CC=C(OCCC(C2=CC=C(Cl)C=C2Cl)CN2C=CN=C2)C=C1
InChi Key	FHKXBOJPHOEMQO-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H17Cl2FN2O/c20-15-1-6-18(19(21)11-15)14(12-24-9-8-23-13-24)7-10-25-17-4-2-16(22)3-5-17/h1-6,8-9,11,13-14H,7,10,12H2
Chemical Name	1-[2-(2,4-dichlorophenyl)-4-(4-fluorophenoxy)butyl]imidazole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	IMB-301 is able to hinder hA3G's degradation by Vif, which leads to hA3G-dependent anti-HIV activity, and hence enhance hA3G's virion encapsidation. IMB-301 attaches itself to hA3G, increases hA3G expression when Vif is present, and hA3G-dependently prevents HIV-1 replication[1].
References	[1]. Ma L, et al. Identification of small molecule compounds targeting the interaction of HIV-1 Vif and human APOBEC3G by virtual screening and biological evaluation. Sci Rep. 2018;8(1):8067. Published 2018 May 23.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (263.67 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6367 mL	13.1836 mL	26.3671 mL
	5 mM	0.5273 mL	2.6367 mL	5.2734 mL
	10 mM	0.2637 mL	1.3184 mL	2.6367 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.