IDT307 is an analog of the organic cationic MPP+ and is the fluorescent substrate of DAT (fluorescent substrate APP+).

Physicochemical Properties

Molecular Formula	C14H17IN2
Molecular Weight	340.20
Exact Mass	340.044
CAS #	1141-41-9
PubChem CID	13227270
Appearance	Yellow to orange solid powder
Melting Point	180-182 °C
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	2
Heavy Atom Count	17
Complexity	199
Defined Atom Stereocenter Count	0
InChi Key	CAMWVBRDIKKGII-UHFFFAOYSA-M
InChi Code	InChI=1S/C14H17N2.HI/c1-15(2)14-6-4-12(5-7-14)13-8-10-16(3)11-9-13;/h4-11H,1-3H3;1H/q+1;/p-1
Chemical Name	N,N-dimethyl-4-(1-methylpyridin-1-ium-4-yl)aniline;iodide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	IDT307, which is transported into the apical membrane (CSF-facing) of CP epithelial cells, is an analog of the organic cationic MPP+ and is susceptible to inhibition by quinine, a PMAT inhibitor [1]. Pmat knockout mice's CP tissues showed a significant 70% reduction in IDT307 uptake and intracellular accumulation [1].
References	[1]. Molecular mechanisms of organic cation and anion transport at the blood‐CSF barrier. 01 April 2018.

Solubility Data

Solubility (In Vitro)

DMSO: 62.5 mg/mL (183.72 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (6.11 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.11 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9394 mL	14.6972 mL	29.3945 mL
	5 mM	0.5879 mL	2.9394 mL	5.8789 mL
	10 mM	0.2939 mL	1.4697 mL	2.9394 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.