Hexa-His is a hexapeptide composed of 6 histidines, which is often used as the metal binding site of fusion proteins.

Physicochemical Properties

Molecular Formula	C36H44N18O7
Molecular Weight	840.850960000001
Exact Mass	840.364
CAS #	64134-30-1
Related CAS #	Hexa-His TFA;1456728-20-3
PubChem CID	10123561
Appearance	White to off-white solid powder
LogP	-5.7
Hydrogen Bond Donor Count	13
Hydrogen Bond Acceptor Count	14
Rotatable Bond Count	23
Heavy Atom Count	61
Complexity	1500
Defined Atom Stereocenter Count	6
SMILES	NC(C(NC(C(NC(C(NC(C(NC(C(NC(C(O)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1)=O)CC1N=CNC=1
InChi Key	XSYUPRQVAHJETO-WPMUBMLPSA-N
InChi Code	InChI=1S/C36H44N18O7/c37-25(1-19-7-38-13-44-19)31(55)50-26(2-20-8-39-14-45-20)32(56)51-27(3-21-9-40-15-46-21)33(57)52-28(4-22-10-41-16-47-22)34(58)53-29(5-23-11-42-17-48-23)35(59)54-30(36(60)61)6-24-12-43-18-49-24/h7-18,25-30H,1-6,37H2,(H,38,44)(H,39,45)(H,40,46)(H,41,47)(H,42,48)(H,43,49)(H,50,55)(H,51,56)(H,52,57)(H,53,58)(H,54,59)(H,60,61)/t25-,26-,27-,28-,29-,30-/m0/s1
Chemical Name	(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-(1H-imidazol-5-yl)propanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Insight into the coordination and the binding sites of Cu(2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53(13):6675-83.

Solubility Data

Solubility (In Vitro)	H2O : ~50 mg/mL (~59.46 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (118.93 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1893 mL	5.9464 mL	11.8927 mL
	5 mM	0.2379 mL	1.1893 mL	2.3785 mL
	10 mM	0.1189 mL	0.5946 mL	1.1893 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.