Hematoporphyrin monomethyl ether is a second-generation porphyrin photosensitizer with the characteristics of single form, high singlet oxygen yield, high selectivity, and low toxicity. It is extensively used in the diagnosis and research of various tumors such as lung cancer and bladder cancer. Glioma and nevus flamingum.

Physicochemical Properties

Molecular Formula	C70H80N8O12
Molecular Weight	1225.43
Exact Mass	612.294
CAS #	148471-91-4
Appearance	Typically exists as solid at room temperature
Density	1.3±0.1 g/cm3
Boiling Point	1123.6±65.0 °C at 760 mmHg
Flash Point	633.3±34.3 °C
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.622
LogP	6.87
SMILES	CC(C1=C(C)C2=NC1=CC1=C(C)C(CCC(=O)O)=C(C=C3C(CCC(=O)O)=C(C)C(C=C4C(C)=C(C(OC)C)C(=C2)N4)=N3)N1)O
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	For photodynamic treatment (PDT), hematoporphyrin monomethyl ether (HMME) is a unique and promising porphyrin-related photosensitizer. In HeLa cells, HMME-PDT can cause both necrosis and apoptosis to cause cell death. HeLa cell-produced ROS, such as hydroxyl and singlet oxygen, are essential for HMME-PDT-induced cell death[2].
References	[1]. Ding X, et al. Hematoporphyrin monomethyl ether photodynamic damage on HeLa cells by means of reactive oxygen species production and cytosolic free calcium concentration elevation. Cancer Lett. 2004;216(1):43-54.

Solubility Data

Solubility (In Vitro)

DMSO : 62.5 mg/mL (51.00 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (1.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.8160 mL	4.0802 mL	8.1604 mL
	5 mM	0.1632 mL	0.8160 mL	1.6321 mL
	10 mM	0.0816 mL	0.4080 mL	0.8160 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.