HUP30 is a vasodilator. HUP30 antagonizes phenylephrine-induced extracellular Ca(2+) influx and mobilization of intracellular stored Ca(2+): this effect is not altered by the presence of ODQ. I(Ba(L)) is blocked by HUP30 in a concentration-dependent and ODQ-independent manner. HUP30 is a vasodilator that stimulates soluble guanylyl cyclase, activates K(+) channels, and blocks extracellular Ca(2+) influx.

Physicochemical Properties

Molecular Formula	C14H15N3O3S
Molecular Weight	305.352
Exact Mass	305.083
Elemental Analysis	C, 55.07; H, 4.95; N, 13.76; O, 15.72; S, 10.50
CAS #	312747-21-0
PubChem CID	739510
Appearance	White to light yellow solid powder
LogP	4.896
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	21
Complexity	409
Defined Atom Stereocenter Count	0
InChi Key	VMAKFFKOZSLHDW-UHFFFAOYSA-N
InChi Code	InChI=1S/C14H15N3O3S/c18-13(9-4-2-1-3-5-9)16-14-15-11-7-6-10(17(19)20)8-12(11)21-14/h6-9H,1-5H2,(H,15,16,18)
Chemical Name	O=C(C1CCCCC1)NC2=NC3=CC=C([N+]([O-])=O)C=C3S2
Synonyms	HUP30; HUP-30; HUP 30;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

HUP30 has an IC50 value of 3.9 μM, which inhibits aortic contraction produced by inhibin [1]. Deoxygen-stimulated aortas have higher cGMP levels when exposed to HUP30 (100 μM) [1]. With an IC50 value of 7.5 μM, HUP30 has an antispasmodic effect on arterial aortic rings contracted by 25/30 mM K+[1]. Ca2+ excitation and extracellular Ca2+ influx are induced by HUP30 (3-100 μM) gyro buffer [1]. Single tail artery myocytes' currents are gyroscoped by HUP30 (10-100 μM) [1].

References

[1]. 2-Aryl- and 2-amido-benzothiazoles as multifunctional vasodilators on rat artery preparations. Eur J Pharmacol. 2013 Aug 15;714(1-3):178-87.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~327.49 mM )

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2749 mL	16.3747 mL	32.7493 mL
	5 mM	0.6550 mL	3.2749 mL	6.5499 mL
	10 mM	0.3275 mL	1.6375 mL	3.2749 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.