HSL-IN-3 (example 42) is a boronic acid analogue and an inhibitor (blocker/antagonist) of hormone-sensitive lipase.

Physicochemical Properties

Molecular Formula	C14H19BO4
Molecular Weight	262.11
Exact Mass	262.137
CAS #	346656-34-6
PubChem CID	10332902
Appearance	Colorless to light yellow liquid
Density	1.1±0.1 g/cm3
Boiling Point	384.8±25.0 °C at 760 mmHg
Flash Point	186.5±23.2 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.499
LogP	1.631
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	19
Complexity	309
Defined Atom Stereocenter Count	0
SMILES	O1B(C2=C([H])C([H])=C([H])C([H])=C2C(=O)OC([H])([H])C([H])([H])[H])OC([H])([H])C(C([H])([H])[H])(C([H])([H])[H])C1([H])[H]
InChi Key	GZHASWCTAWNFFW-UHFFFAOYSA-N
InChi Code	InChI=1S/C14H19BO4/c1-4-17-13(16)11-7-5-6-8-12(11)15-18-9-14(2,3)10-19-15/h5-8H,4,9-10H2,1-3H3
Chemical Name	ethyl 2-(5,5-dimethyl-1,3,2-dioxaborinan-2-yl)benzoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	HSL[1].
References	[1]. PHARMACEUTICAL USE OF BORONIC ACIDS AND ESTERS THEREOF. US7037905.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (381.52 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (7.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (7.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8152 mL	19.0760 mL	38.1519 mL
	5 mM	0.7630 mL	3.8152 mL	7.6304 mL
	10 mM	0.3815 mL	1.9076 mL	3.8152 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.