HKSOX-1r (5/6-mixture) is a fluorescent probe used to image and detect endogenous superoxide in living cells and in vivo. HKSOX-1r (5/6-mixture) has excellent selectivity and sensitivity towards superoxide anion radicals.

Physicochemical Properties

Molecular Formula	C29H15F10NO14S2
Molecular Weight	855.54
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	HKSOX-1r 5/6-mixture (2 μM; 30 min) detects O2?-formation generated by mitochondrial respiration inhibitors in HCT116, BV-2, and RAW264.7 cells very quickly and sensitively [1].
ln Vivo	HKSOX-1r 5/6-mixture (10 μM; 20 min) Zebrafish embryos treated with PMA or Antimycin A showed a distinct pattern of fluorescence [1].
References	[1]. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc. 2015 Jun 3;137(21):6837-43.

Solubility Data

Solubility (In Vitro)

DMSO :~200 mg/mL (~233.77 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (5.84 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1689 mL	5.8443 mL	11.6885 mL
	5 mM	0.2338 mL	1.1689 mL	2.3377 mL
	10 mM	0.1169 mL	0.5844 mL	1.1689 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.