HKSOX-1m (5/6-mixture) is an O2 fluorescent probe targeting mitochondria (Ex/Em=509/534 nm; green). It has excellent selectivity and is effective in cells over a wide range of pH values. It is still effective even in the presence of large amounts of strong oxidizing and reducing agents.

Physicochemical Properties

Molecular Formula	C49H37CL2F10N2O10PS2
Molecular Weight	1169.82
Exact Mass	2339.169
CAS #	1786411-19-5
Related CAS #	HKSOX-1m (5/6-mixture) (hydrobromide)
PubChem CID	162642064
Appearance	Typically exists as solid at room temperature
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	44
Rotatable Bond Count	26
Heavy Atom Count	152
Complexity	4150
Defined Atom Stereocenter Count	0
InChi Key	GSKUONHKIPASJJ-UHFFFAOYSA-L
InChi Code	InChI=1S/2C49H36F10N2O10PS2.4ClH/c50-37-27-35-41(39(52)43(37)70-73(64,65)48(54,55)56)68-42-36(28-38(51)44(40(42)53)71-74(66,67)49(57,58)59)47(35)34-19-18-29(26-33(34)46(63)69-47)45(62)61-23-21-60(22-24-61)20-10-11-25-72(30-12-4-1-5-13-30,31-14-6-2-7-15-31)32-16-8-3-9-17-32;50-37-27-35-41(39(52)43(37)70-73(64,65)48(54,55)56)68-42-36(28-38(51)44(40(42)53)71-74(66,67)49(57,58)59)47(35)34-26-29(18-19-33(34)46(63)69-47)45(62)61-23-21-60(22-24-61)20-10-11-25-72(30-12-4-1-5-13-30,31-14-6-2-7-15-31)32-16-8-3-9-17-32;;;;/h2*1-9,12-19,26-28H,10-11,20-25H2;4*1H/q2*+1;;;;/p-2
Chemical Name	triphenyl-[4-[4-[2',4',5',7'-tetrafluoro-1-oxo-3',6'-bis(trifluoromethylsulfonyloxy)spiro[2-benzofuran-3,9'-xanthene]-5-carbonyl]piperazin-1-yl]butyl]phosphanium;triphenyl-[4-[4-[2',4',5',7'-tetrafluoro-3-oxo-3',6'-bis(trifluoromethylsulfonyloxy)spiro[2-benzofuran-1,9'-xanthene]-5-carbonyl]piperazin-1-yl]butyl]phosphanium;dichloride;dihydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	At very low laser power output conditions, HKSOX-1m (5/6-mixture) (10 μM) sensitively captures basal signals in differentiated human THP-1 cells and mitochondria stimulated by Antimycin A (1 μM). The signal of O2 (with an intensity of 514 nm excitation wavelength of Zeiss LSM 510 Meta being 1%), is captured.
References	[1]. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc . 2015 Jun 3;137(21):6837-43.

Solubility Data

Solubility (In Vitro)

DMSO: 55 mg/mL (47.02 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.75 mg/mL (2.35 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.8548 mL	4.2742 mL	8.5483 mL
	5 mM	0.1710 mL	0.8548 mL	1.7097 mL
	10 mM	0.0855 mL	0.4274 mL	0.8548 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.