HKOCl-3 is a highly sensitive and selective fluorescent probe for detecting hypochlorous acid. Ex: 490 nm; Em 527 nm.

Physicochemical Properties

Molecular Formula	C26H14CL2O6
Molecular Weight	493.29176568985
Exact Mass	492.016
CAS #	2031170-80-4
PubChem CID	122646982
Appearance	Yellow to orange solid powder
LogP	6.2
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	2
Heavy Atom Count	34
Complexity	770
Defined Atom Stereocenter Count	0
SMILES	C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(OC1=CC(Cl)=C(O)C(Cl)=C1)=C3)C1=C(C=CC=C1)C(=O)O2
InChi Key	OWWYBZBGVRLHND-UHFFFAOYSA-N
InChi Code	InChI=1S/C26H14Cl2O6/c27-20-10-15(11-21(28)24(20)30)32-14-6-8-19-23(12-14)33-22-9-13(29)5-7-18(22)26(19)17-4-2-1-3-16(17)25(31)34-26/h1-12,29-30H
Chemical Name	3'-(3,5-dichloro-4-hydroxyphenoxy)-6'-hydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Ex: 490 nm; Em 527 nm
ln Vitro	HKOCl-3 (10 μM) exhibits a greater degree of florescence with an increase in hypochlorous acid concentration[1]. When HKOCl-3 (10 μM) is treated, the compound's fluorescence intensity increases significantly more than when other ROS (10 μM) and RNS (100 μM) are treated [1].
References	[1]. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (202.72 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 5 mg/mL (10.14 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0272 mL	10.1360 mL	20.2721 mL
	5 mM	0.4054 mL	2.0272 mL	4.0544 mL
	10 mM	0.2027 mL	1.0136 mL	2.0272 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.