HDAC6-IN-36 (compound 11d) is a HDAC6 inhibitor with IC50 of 8.64 nM. HDAC6-IN-36 induces neurite outgrowth in PC12 cells without causing toxic effects.

Physicochemical Properties

Molecular Formula	C30H29CLN4O3
Molecular Weight	529.03
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	HDAC1 1284 nM (IC50); HDAC6 8.64 nM (IC50)
ln Vitro	HDAC6-IN-36 (1 μM, 24 h) can increase the acetylation levels of histone H3 and ɑ-tubulin in PC12 cell lines. HDAC6-IN-36 (1-10 μM, 48 h) can stimulate neurite outgrowth in PC12 cells under the induction of NGF (20 ng/mL).
Cell Assay	Western Blot Analysis[1] Cell Types: PC12 cell Tested Tested Concentrations: 0.1, 0.5 and 1 μM Incubation Duration: 24 h Experimental Results: Increased the acetylation level of α-tubulin in a dose-dependent manner.
References	[1]. Re-exploration of tetrahydro-β-carboline scaffold: Discovery of selective histone deacetylase 6 inhibitors with neurite outgrowth-promoting and neuroprotective activities. Bioorg Med Chem Lett. 2024.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8903 mL	9.4513 mL	18.9025 mL
	5 mM	0.3781 mL	1.8903 mL	3.7805 mL
	10 mM	0.1890 mL	0.9451 mL	1.8903 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.