Physicochemical Properties
| Molecular Formula | C55H68F3N9O19 |
| Molecular Weight | 1216.17 |
| Related CAS # | HA Peptide;92000-76-5 |
| Appearance | White to off-white solid powder |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | In neutral pH, tagged proteins are frequently isolated from cell culture supernatants and cell lysates using HA Peptide, a highly immunoreactive label. As such, Western blot provides a straightforward method of detection, making them useful instruments for coimmunoprecipitation. The fusion partner protein's biological activity and function are unlikely to be affected by the tiny size of HA Peptide. The HA Peptide is a useful tool for isolating, purifying, detecting, and tracking proteins of interest. It is derived from human influenza hemagglutinin (HA), which corresponds to amino acids 98–106. It is a powerfully immunoreactive epitope. Anti-HA monoclonal antibodies that are highly selective and covalently bound on resin can be used to isolate recombinant HA-tagged proteins. Using TBS containing 1 mg/mL of the HA epitope, HA-tagged proteins can be gently eluted. Three alternatives exist for chemical elution as well: 50 mM NaOH, 3 M NaSCN, and 0.1 M glycine (pH 2-2.8) [1]. For T7 promoter-driven expression in E. coli, even in the absence of transcriptionally active T7 RNAP, the nucleotide sequence encoding the N-terminal HA Peptide in mammalian expression vectors is crucial [2]. According to research findings, caspase 3/7 cleaves the HA peptide, which leaves the immune response completely destroyed. Studies on apoptotic pathways linked to cell death have shown that using HA-tagged proteins and constructs may result in significant artifacts [3]. |
| References |
[1]. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093. [2]. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6. [3]. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8. |
Solubility Data
| Solubility (In Vitro) |
DMSO :≥ 100 mg/mL (~82.23 mM) H2O :~50 mg/mL (~41.11 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (1.03 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (1.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.25 mg/mL (1.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8223 mL | 4.1113 mL | 8.2225 mL | |
| 5 mM | 0.1645 mL | 0.8223 mL | 1.6445 mL | |
| 10 mM | 0.0822 mL | 0.4111 mL | 0.8223 mL |