H-Tyr-Ala-OH (Tyrosylalanine) is a dipeptide containing L-tyrosine and L-alanine.

Physicochemical Properties

Molecular Formula	C12H16N2O4
Molecular Weight	252.27
Exact Mass	252.111
CAS #	730-08-5
PubChem CID	5496455
Sequence	H-Tyr-Ala-OH; L-tyrosyl-L-alanine
SequenceShortening	YA
Appearance	White to off-white solid powder
Density	1.315g/cm3
Boiling Point	577.8ºC at 760 mmHg
Melting Point	230 ºC (decomp)
Flash Point	303.3ºC
Index of Refraction	1.591
LogP	0.942
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	18
Complexity	301
Defined Atom Stereocenter Count	2
SMILES	C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)N
InChi Key	NLKUJNGEGZDXGO-XVKPBYJWSA-N
InChi Code	InChI=1S/C12H16N2O4/c1-7(12(17)18)14-11(16)10(13)6-8-2-4-9(15)5-3-8/h2-5,7,10,15H,6,13H2,1H3,(H,14,16)(H,17,18)/t7-,10-/m0/s1
Chemical Name	(2S)-2-[[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]propanoic acid
Synonyms	Tyr-Ala; 730-08-5; tyrosylalanine; L-tyrosyl-L-alanine; Tyrosyl-Alanine; L-Tyr-L-Ala; CHEBI:74879; (2S)-2-[[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]propanoic acid;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Bioactive peptide
ln Vitro	H-Tyr-Ala-OH (Tyrosylalanine) exhibited cytochrome C reducing activity. In an in vitro assay, it was found to reduce cytochrome C, as indicated by a change in absorbance at a specific wavelength, reflecting its ability to participate in electron transfer processes [2]
Enzyme Assay	For assessing cytochrome C reducing activity of H-Tyr-Ala-OH (Tyrosylalanine), a reaction mixture containing cytochrome C and the dipeptide was prepared. The mixture was incubated under controlled conditions, and the reduction of cytochrome C was monitored by measuring the change in absorbance over time at a specific wavelength. The extent of reduction was used to evaluate the activity of the dipeptide [2] Spectroscopic and Structural Analysis (included in Additional Info as per context): H-Tyr-Ala-OH (Tyrosylalanine) was subjected to solid-state IR-LD spectroscopy to analyze its vibrational properties. Quantum chemical calculations and vibrational analysis were also performed to elucidate its structural characteristics, including bond vibrations and molecular geometry in the solid state [1]
References	[1]. Spectroscopic and structural elucidation of L-tyrosine-containing dipeptides valyl-tyrosine and tyrosyl-alanine: solid-state IR-LD spectroscopy, quantum chemical calculations and vibrational analysis. Spectrochim Acta A Mol Biom. [2]. A new cytochrome C reducing dipeptide. J Natl Med Assoc. 1990 Feb;82(2):113-6.
Additional Infomation	H-Tyr-Ala-OH (Tyrosylalanine) is a dipeptide containing L-tyrosine. Its structural and spectroscopic properties were investigated using IR-LD spectroscopy combined with quantum chemical calculations to understand its molecular structure and vibrational behavior [1] ; It was identified as a dipeptide with cytochrome C reducing activity, contributing to redox processes in vitro [2] Tyr-Ala is a dipeptide formed from L-tyrosine and L-alanine residues. It has a role as a metabolite. Tyr-Ala has been reported in Brassica napus and Aeromonas veronii with data available.

Solubility Data

Solubility (In Vitro)	H2O: 125 mg/mL (495.50 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (396.40 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9640 mL	19.8200 mL	39.6401 mL
	5 mM	0.7928 mL	3.9640 mL	7.9280 mL
	10 mM	0.3964 mL	1.9820 mL	3.9640 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.