Guancydine (Guancidine) is an anti-hypertensive (blood pressure lowering) agent.

Physicochemical Properties

Molecular Formula	C7H14N4
Molecular Weight	154.21300
Exact Mass	154.122
CAS #	1113-10-6
PubChem CID	14211
Appearance	White to off-white solid powder
Density	1.02g/cm3
Boiling Point	239.3ºC at 760 mmHg
Flash Point	98.5ºC
Vapour Pressure	0.0404mmHg at 25°C
Index of Refraction	1.504
LogP	1.651
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	3
Heavy Atom Count	11
Complexity	196
Defined Atom Stereocenter Count	0
InChi Key	VZVGEDRCVUKSEL-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H14N4/c1-4-7(2,3)11-6(9)10-5-8/h4H2,1-3H3,(H3,9,10,11)
Chemical Name	1-cyano-2-(2-methylbutan-2-yl)guanidine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Guancydine: a new antihypertensive agent. Use with quinethazone and guanethidine or propranolol. Ann Intern Med. 1972 Apr;76(4):579-85.
Additional Infomation	Guancidine is a member of guanidines.

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 125 mg/mL (~810.58 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (13.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (13.49 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (13.49 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.4847 mL	32.4233 mL	64.8466 mL
	5 mM	1.2969 mL	6.4847 mL	12.9693 mL
	10 mM	0.6485 mL	3.2423 mL	6.4847 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.