Golgicide A-1 (GCA-1) is the less active cis diastereomer of Golgicide A (GCA), which weakly inhibits mosquito reproduction.

Physicochemical Properties

Molecular Formula	C17H14F2N2
Molecular Weight	284.30
Exact Mass	284.112
CAS #	1394285-49-4
Related CAS #	Golgicide A;1139889-93-2;Golgicide A-2;1394285-50-7;(Rac)-Golgicide A;1005036-73-6
PubChem CID	1085377
Appearance	White to light yellow solid powder
LogP	3.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	21
Complexity	414
Defined Atom Stereocenter Count	3
SMILES	C1C=C[C@H]2[C@@H]1[C@H](NC3=C2C=C(C=C3F)F)C4=CN=CC=C4
InChi Key	NJZHEQOUHLZCOX-WOSRLPQWSA-N
InChi Code	InChI=1S/C17H14F2N2/c18-11-7-14-12-4-1-5-13(12)16(10-3-2-6-20-9-10)21-17(14)15(19)8-11/h1-4,6-9,12-13,16,21H,5H2/t12-,13+,16+/m0/s1
Chemical Name	(3aR,4S,9bS)-6,8-difluoro-4-pyridin-3-yl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In the Aedes aegypti and Anopheles stephensi larvae experiment, glyphicide A-1 (GCA-1) exhibits negligible to no cytotoxicity[1].
References	[1]. Daniel J Mack, et al. Distinct biological effects of golgicide a derivatives on larval and adult mosquitoes. Bioorg Med Chem Lett. 2012 Aug 15;22(16):5177-81.
Additional Infomation	(3aR,4S,9bS)-golgicide A is a 6,8-difluoro-4-(pyridin-3-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline that has 3aR,4S,9bS configuration. It is an enantiomer of a (3aS,4R,9bR)-golgicide A.

Solubility Data

Solubility (In Vitro)

DMSO: 110 mg/mL (386.92 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 5.5 mg/mL (19.35 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5.5 mg/mL (19.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5174 mL	17.5871 mL	35.1741 mL
	5 mM	0.7035 mL	3.5174 mL	7.0348 mL
	10 mM	0.3517 mL	1.7587 mL	3.5174 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.