GW814408X is a kinase chemogenetic group (KCGS) compound that inhibits AURKC kinases involved in cell cycle progression, checkpoint regulation, and cell division. GW814408X exhibits cell line-dependent toxicity, such as cytotoxicity to HeLa cells. GW814408X acts as a protein kinase inhibitor across ATP-dependent and -independent luciferases, with potential effects on Fluc reporter gene assays.

Physicochemical Properties

Molecular Formula	C19H16N6O
Molecular Weight	344.37
CAS #	886599-05-9
SMILES	C1=NC=CC(C=NNC2N=CN=C3C(C4=CC=CC(OC)=C4)=CNC3=2)=C1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. The Kinase Chemogenomic Set (KCGS): An Open Science Resource for Kinase Vulnerability Identification. Int J Mol Sci. 2021 Jan 8;22(2):566. [2]. Profile of the GSK published protein kinase inhibitor set across ATP-dependent and-independent luciferases: implications for reporter-gene assays. PLoS One. 2013;8(3):e57888.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9039 mL	14.5193 mL	29.0385 mL
	5 mM	0.5808 mL	2.9039 mL	5.8077 mL
	10 mM	0.2904 mL	1.4519 mL	2.9039 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.