GW549390X is a dual inhibitor of Fluc and VEGFR2 with IC50 of 0.26 μM and 1.2 μM, respectively. GW549390X can bind to the ATP pocket of FLuc through the aniline side chain and is an ATP-competitive inhibitor of Fluc. GW549390X is a protein kinase inhibitor across ATP-dependent and -independent luciferases, and has potential effects on Fluc reporter gene assays.

Physicochemical Properties

Molecular Formula	C15H12N2O
Molecular Weight	236.27
CAS #	135307-33-4
SMILES	C1(NC2=NC=C(C3=CC=CC=C3)O2)=CC=CC=C1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Profile of the GSK published protein kinase inhibitor set across ATP-dependent and-independent luciferases: implications for reporter-gene assays. PLoS One. 2013;8(3):e57888.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2324 mL	21.1622 mL	42.3245 mL
	5 mM	0.8465 mL	4.2324 mL	8.4649 mL
	10 mM	0.4232 mL	2.1162 mL	4.2324 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.