GSK8573 is an inactive control compound for GSK2801, an acetyl-lysine competitive inhibitor of the BAZ2A and BAZ2B bromodomains. GSK8573 has binding activity to BRD9 with Kd of 1.04 μM and is inactive against BAZ2A/B and other bromodomain families. GSK8573 can be used as a structurally related negative control (NC) compound in biological experiments.

Physicochemical Properties

Molecular Formula	C20H21NO3
Molecular Weight	323.385645627975
Exact Mass	323.152
CAS #	1693766-04-9
Related CAS #	1693766-04-9 GSK8573
PubChem CID	121230107
Appearance	Light yellow to yellow solid powder
Density	1.1±0.1 g/cm3
Index of Refraction	1.569
LogP	4.44
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	6
Heavy Atom Count	24
Complexity	427
Defined Atom Stereocenter Count	0
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	GSK8573 (1.0 μM) is inert against BAZ2A/B and other bromodomain families, but has binding activity to BRD9 with a Kd value of 1.04 μM [1].
References	[1]. Discovery and Characterization of GSK2801, a Selective Chemical Probe for the Bromodomains BAZ2A and BAZ2B. J Med Chem. 2016 Feb 25;59(4):1410-24.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~77.31 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0922 mL	15.4612 mL	30.9224 mL
	5 mM	0.6184 mL	3.0922 mL	6.1845 mL
	10 mM	0.3092 mL	1.5461 mL	3.0922 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.