DG70 (GSK1733953A) is a benzidine, a respiratory inhibitor of Mycobacterium tuberculosis (TB), inhibiting MenG activity with IC50 of 2.6 ± 0.6 μM. DG70 inhibits catalytic methylation by Mycobacterium tuberculosis (TB) desmenaquinone methyltransferase. DG70 may be utilized in tuberculosis (TB)-related research.

Physicochemical Properties

Molecular Formula	C21H17CLFNO3
Molecular Weight	385.815988302231
Exact Mass	385.09
Elemental Analysis	C, 65.38; H, 4.44; Cl, 9.19; F, 4.92; N, 3.63; O, 12.44
CAS #	930470-97-6
Related CAS #	930470-97-6 GSK1733953A
PubChem CID	17555992
Appearance	Solid powder
LogP	5.1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	5
Heavy Atom Count	27
Complexity	486
Defined Atom Stereocenter Count	0
InChi Key	RWSCJEILSRMTEH-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H17ClFNO3/c1-26-16-7-3-13(4-8-16)18-12-15(6-10-20(18)27-2)24-21(25)17-9-5-14(23)11-19(17)22/h3-12H,1-2H3,(H,24,25)
Chemical Name	2-chloro-4-fluoro-N-[4-methoxy-3-(4-methoxyphenyl)phenyl]benzamide
Synonyms	GSK1733953A; GSK-1733953A; GSK 1733953A; DG70; DG-70; DG 70;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Mycobacterial MenG: Partial Purification, Characterization, and Inhibition. ACS Infect Dis. 2022 Dec 9;8(12):2430-2440. [2]. Thompson loop: opportunities for antitubercular drug design by targeting the weak spot in demethylmenaquinone methyltransferase protein. RSC Adv. 2020 Jun 19;10(39):23466-23483. [3]. A Novel Small-Molecule Inhibitor of the Mycobacterium tuberculosis Demethylmenaquinone Methyltransferase MenG Is Bactericidal to Both Growing and Nutritionally Deprived Persister Cells. mBio. 2017 Feb 14;8(1):e02022-16.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~259.19 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.48 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5919 mL	12.9594 mL	25.9188 mL
	5 mM	0.5184 mL	2.5919 mL	5.1838 mL
	10 mM	0.2592 mL	1.2959 mL	2.5919 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.