GCN2-IN-1 (A-92) is a potent inhibitor of general regulatory repressor protein kinase 2 (GCN2) with IC50 of <0.3 μM in enzyme assays and IC50 of 0.3-3 μM in cell assays.

Physicochemical Properties

Molecular Formula	C19H18N10O
Molecular Weight	402.412621021271
Exact Mass	402.166
CAS #	1448693-69-3
PubChem CID	89720372
Appearance	Light yellow to yellow solid powder
Density	1.7±0.1 g/cm3
Boiling Point	760.0±70.0 °C at 760 mmHg
Flash Point	413.4±35.7 °C
Vapour Pressure	0.0±2.6 mmHg at 25°C
Index of Refraction	1.902
LogP	0.87
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	4
Heavy Atom Count	30
Complexity	592
Defined Atom Stereocenter Count	0
InChi Key	CFNFLNGJQOHNPR-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H18N10O/c1-2-15(7-16-12(1)8-21-25-16)29-18-17(26-27-29)10-20-19(24-18)23-13-9-22-28(11-13)14-3-5-30-6-4-14/h1-2,7-11,14H,3-6H2,(H,21,25)(H,20,23,24)
Chemical Name	3-(1H-indazol-6-yl)-N-[1-(oxan-4-yl)pyrazol-4-yl]triazolo[4,5-d]pyrimidin-5-amine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	GCN2-IN-1 (Compound A-92) is an example of GCN2 that can be employed in real-world chemical processing chemistry [1].
References	[1]. Triazolo[4,5-d]pyrimidine derivatives. WO2013110309A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~60 mg/mL (~149.10 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4850 mL	12.4251 mL	24.8503 mL
	5 mM	0.4970 mL	2.4850 mL	4.9701 mL
	10 mM	0.2485 mL	1.2425 mL	2.4850 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.