G7-18NATE is a peptide inhibitor of Grb7. G7-18NATE binds to the Grb7-SH2 domain with micromolar affinity (KD = 18.1 μM). G7-18NATE inhibits cell proliferation, motility, cell invasion, and 3D culture formation in multiple cancer cell lines.

Physicochemical Properties

Molecular Formula	C67H80N14O19S
Molecular Weight	1417.50
CAS #	936728-13-1
Related CAS #	G7-18NATE TFA
SMILES	C([C@@H]1NC(CSC[C@H](NC([C@]2([H])CCCN2C([C@@H](NC([C@@]([H])(NC([C@@H](NC([C@@H](NC([C@@H](NC(CNC([C@@H](NC([C@@H](NC1=O)CC1C=CC=CC=1)=O)CCC(=O)O)=O)=O)CC1C=CC(=CC=1)O)=O)CC(=O)O)=O)CC(=O)N)=O)[C@H](O)C)=O)CC1C=CC=CC=1)=O)=O)C(=O)N)=O)C1=CNC2=CC=CC=C12
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	G7-18NATE (10 μM, 24 h) inhibits the migration of MDA-MB-468 cells[2].
References	[1]. Evaluation of Cyclic Peptide Inhibitors of the Grb7 Breast Cancer Target: Small Change in Cargo Results in Large Change in Cellular Activity. Molecules. 2019 Oct 17;24(20):3739. [2]. GRB7 is required for triple-negative breast cancer cell invasion and survival. Breast Cancer Res Treat. 2012 Jun;133(2):607-15.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.7055 mL	3.5273 mL	7.0547 mL
	5 mM	0.1411 mL	0.7055 mL	1.4109 mL
	10 mM	0.0705 mL	0.3527 mL	0.7055 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.