G15 is a high-affinity and selective G-protein-coupled estrogen receptor (GPER/GPR30) antagonist (inhibitor) with Ki of 20 nM.

Physicochemical Properties

Molecular Formula	C19H16BRNO2
Molecular Weight	370.246
Exact Mass	369.036
CAS #	1161002-05-6
PubChem CID	7433743
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	462.7±45.0 °C at 760 mmHg
Flash Point	233.7±28.7 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.649
LogP	4.49
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	23
Complexity	484
Defined Atom Stereocenter Count	3
SMILES	C1C=C[C@@H]2[C@H]1[C@@H](NC3=CC=CC=C23)C4=CC5=C(C=C4Br)OCO5
InChi Key	YOLTZIVRJAPVPH-MJLGCCKJSA-N
InChi Code	InChI=1S/C19H16BrNO2/c20-15-9-18-17(22-10-23-18)8-14(15)19-13-6-3-5-11(13)12-4-1-2-7-16(12)21-19/h1-5,7-9,11,13,19,21H,6,10H2/t11-,13-,19+/m0/s1
Chemical Name	(3aS,4R,9bR)-4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline
Synonyms	G 15; G15; G-15
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	A549 and H1793 cell lines' GPER-mediated proliferation is inhibited by G15 (0.1–10 μM; 2 days) in response to 17β-estradiol (E2) [1]. G15 inhibits H1793 and A549 cell lines at 1 μM for 48 hours.
ln Vivo	In formate-induced adenocarcinoma inflammation, G15 (1.46 mg/kg; ih; twice weekly for 14 weeks) decreases the increased nodule number and tumor index in E2 or G1 group tumors [1].
Cell Assay	Cell proliferation assay [1] Cell Types: A549, H1793 cell lines Tested Concentrations: 0.1, 1, 10μM (combined with 10nM E2) Incubation Duration: 2 days Experimental Results: Inhibition of GPER reaction. -E2 stimulus-mediated proliferation. Western Blot Analysis [1] Cell Types: A549, H1793 cell lines Tested Concentrations: 1 μM (combined with 10 nM E2 and 10 nM G1) Incubation Duration: 48 hrs (hours) Experimental Results: Inhibition of E2 and G1 stimulated GPER responses.
Animal Protocol	Animal/Disease Models: 4weeks old female Kunming mice (urethane-induced adenocarcinoma) [1] Doses: 1.46 mg/kg (combined with E2, 0.09 mg/kg and Fulvestrant (Ful), 2.4 mg/kg ) Method of Route of Administration: subcutaneous injection; twice a week for 14 weeks. Experimental Results: The number of tumor nodules in the E2+Ful+G15 group was diminished.
References	[1]. G-Protein-Coupled Estrogen Receptor Antagonist G15 Decreases Estrogen-Induced Development of Non-Small Cell Lung Cancer. Oncol Res. 2019 Feb 21;27(3):283-292. [2]. Estrogen Signaling in ERα-Negative Breast Cancer: ERβ and GPER. Front Endocrinol (Lausanne). 2019 Jan 9;9:781.

Solubility Data

Solubility (In Vitro)

DMSO : ~41.67 mg/mL (~112.55 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7009 mL	13.5044 mL	27.0088 mL
	5 mM	0.5402 mL	2.7009 mL	5.4018 mL
	10 mM	0.2701 mL	1.3504 mL	2.7009 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.