Fucoidan is a bioactive polysaccharide that is a potent inhibitor of alpha-amylase and alpha-glucosidase. Has anticoagulant, antitumor, antioxidant and antilipid activities.

Physicochemical Properties

Molecular Formula	C7H14O7S
Molecular Weight	242.2469
Exact Mass	242.046
CAS #	9072-19-9
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Fucoidan is a biologically active polysaccharide found in great abundance in brown marine algae[1]. Fucoidan extracted from A. nodosum is a more potent inhibitor of α-glucosidase, with an IC50 ranging from 0.013 to 0.047 mg/mL, than the inhibition by Fucoidan extracted from F. vesiculosus (IC50=0.049 mg/mL). Fucoidan extracted from F. vesiculosus does not inhibit α-amylase activity, while Fucoidan from A. nodosum decreases α-amylase activity by 7-100% at 5 mg/mL depending upon the algae harvest period. Fucoidan from A. nodosum inhibits α-amylase with an IC50 of 0.12-4.64 mg/mL[1]. Fucoidan is a fucose-rich sulfated polysaccharide typically found in the cell wall of marine algae but also recently isolated from terrestrial sources. Fucoidan exhibits an attractive therapeutic potential against a wide array of metabolic diseases associated with oxidative stress[2].
ln Vivo	While three injections of 10 mg/kg/body weight of fumoidan dramatically diminish tumor development and metastasis, a single injection of 25 mg/kg/body does not prevent the proliferation of tumor cells[3].
Animal Protocol	Animal/Disease Models: Mice[3] Doses: 10-400 mg/kg Route of Administration: intraperitoneal (ip) injection; followed by total-body irradiation Experimental Results: The mice injected with 100 mg/kg demonstrated the best survival rate at 30 days post-irradiation.
References	[1]. Alpha-amylase and alpha-glucosidase inhibition is differentially modulated by fucoidan obtained from Fucus vesiculosus and Ascophyllum nodosum.Phytochemistry. 2014 Feb;98:27-33. [2]. Antioxidant and Antisteatotic Activities of Fucoidan Fractions from Marine and Terrestrial Sources. Molecules. 2021 Jul 24;26(15):4467. [3]. Fucoidan and cancer: a multifunctional molecule with anti-tumor potential. Mar Drugs. 2015 Apr 14;13(4):2327-46.

Solubility Data

Solubility (In Vitro)	H2O: 10 mg/mL DMSO: < 1 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 33.33 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1280 mL	20.6398 mL	41.2797 mL
	5 mM	0.8256 mL	4.1280 mL	8.2559 mL
	10 mM	0.4128 mL	2.0640 mL	4.1280 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.