Fodipir is the bioactive metabolite of mangafodipir and can participate in mangafodipir-mediated cell protection by preventing 7β-antihydroxycholesterol-induced apoptosis.

Physicochemical Properties

Molecular Formula	C22H32N4O14P2
Molecular Weight	638.45548
Exact Mass	638.139
CAS #	118248-91-2
PubChem CID	60683
Appearance	Light yellow to yellow solid powder
Density	1.657g/cm3
Boiling Point	1049.1ºC at 760mmHg
Flash Point	588.3ºC
Vapour Pressure	0mmHg at 25°C
Index of Refraction	1.655
LogP	0.132
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	18
Rotatable Bond Count	17
Heavy Atom Count	42
Complexity	907
Defined Atom Stereocenter Count	0
InChi Key	SQKUFYLUXROIFM-UHFFFAOYSA-N
InChi Code	InChI=1S/C22H32N4O14P2/c1-13-21(31)17(15(5-23-13)11-39-41(33,34)35)7-25(9-19(27)28)3-4-26(10-20(29)30)8-18-16(12-40-42(36,37)38)6-24-14(2)22(18)32/h5-6,31-32H,3-4,7-12H2,1-2H3,(H,27,28)(H,29,30)(H2,33,34,35)(H2,36,37,38)
Chemical Name	2-[2-[carboxymethyl-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methyl]amino]ethyl-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methyl]amino]acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Mangafodipir's active metabolite, fodipir, plays a role in mangafodipir-mediated cytoprotection against cell death caused by 7β-hydroxycholesterol. After 8 hours of therapy, fodipir (Dp-dp; dipyridine diphosphate; 100 μM) demonstrated optimum cytoprotective benefits against 7β-OH-mediated cell death. Additionally, fodipir decreases lysosomal membrane permeabilization (LMP) and ROS generation in cells caused by 7β-OH [1].

References

[1]. Fodipir and its dephosphorylated derivative dipyridoxyl ethyldiamine are involved in mangafodipir-mediated cytoprotection against 7β-hydroxycholesterol-induced cell death. Pharmacology. 2013;92(3-4):182-6.

Solubility Data

Solubility (In Vitro)	H2O : ~18.18 mg/mL (~28.47 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 33.33 mg/mL (52.20 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5663 mL	7.8313 mL	15.6627 mL
	5 mM	0.3133 mL	1.5663 mL	3.1325 mL
	10 mM	0.1566 mL	0.7831 mL	1.5663 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.