Fmoc-Gly-Gly-Phe-OH is a cleavable (degradable) ADC (Antibody-drug conjugate) linker used for the synthesis of antibody active molecule conjugates (ADCs).

Physicochemical Properties

Molecular Formula	C28H27N3O6
Molecular Weight	501.53
Exact Mass	501.189
CAS #	160036-44-2
Related CAS #	Fmoc-Gly-Gly-Phe-OH;160036-44-2
PubChem CID	14598599
Appearance	White to off-white solid powder
LogP	3.3
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	11
Heavy Atom Count	37
Complexity	792
Defined Atom Stereocenter Count	1
SMILES	C1C2=C(C3=C(C2COC(NCC(NCC(N[C@H](C(O)=O)CC2=CC=CC=C2)=O)=O)=O)C=CC=C3)C=CC=1
InChi Key	UFGUUZVZUIXKQZ-DEOSSOPVSA-N
InChi Code	InChI=1S/C28H27N3O6/c32-25(29-16-26(33)31-24(27(34)35)14-18-8-2-1-3-9-18)15-30-28(36)37-17-23-21-12-6-4-10-19(21)20-11-5-7-13-22(20)23/h1-13,23-24H,14-17H2,(H,29,32)(H,30,36)(H,31,33)(H,34,35)/t24-/m0/s1
Chemical Name	(2S)-2-[[2-[[2-(9H-fluoren-9-ylmethoxycarbonylamino)acetyl]amino]acetyl]amino]-3-phenylpropanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Cleavable Linker
References	[1]. Antibody-drug conjugate. Patent WO2014057687A1.

Solubility Data

Solubility (In Vitro)

DMSO : 240 mg/mL (478.54 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 6 mg/mL (11.96 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 60.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 6 mg/mL (11.96 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 60.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 6 mg/mL (11.96 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 60.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9939 mL	9.9695 mL	19.9390 mL
	5 mM	0.3988 mL	1.9939 mL	3.9878 mL
	10 mM	0.1994 mL	0.9969 mL	1.9939 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.