Fmoc-Gly-Gly-D-Phe-OtBu is a widely used linker for cleavable Antibody-drug conjugates (ADCs). Fmoc-Gly-Gly-D-Phe-OtBu is the R-enantiomer of Fmoc-Gly-Gly-Phe-OtBu.

Physicochemical Properties

Molecular Formula	C32H35N3O6
Molecular Weight	557.64
Related CAS #	Fmoc-Gly-Gly-Phe-OtBu;236426-37-2
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Cleavable Linker
References	[1]. Synthesis and evaluation of the physicochemical properties of esterase-sensitive cyclic prodrugs of opioid peptides using an (acyloxy)alkoxy linker. J Pept Res. 1999 Apr;53(4):393-402.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~179.33 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (8.97 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.7933 mL	8.9664 mL	17.9327 mL
	5 mM	0.3587 mL	1.7933 mL	3.5865 mL
	10 mM	0.1793 mL	0.8966 mL	1.7933 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.