Fluorescent DOTAP is a cationic lipid useful in studying nucleic acid and protein delivery.

Physicochemical Properties

Molecular Formula	C36H60CLN5O7
Molecular Weight	710.343909263611
Exact Mass	709.418
CAS #	1010076-97-7
PubChem CID	136212472
Appearance	Brown to reddish brown solid powder
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	29
Heavy Atom Count	49
Complexity	923
Defined Atom Stereocenter Count	0
SMILES	[Cl-].O(C(CCCCCNC1=CC=C(C2C1=NON=2)[N+](=O)[O-])=O)C(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C
InChi Key	XDKRQAPVOCOOGF-USGGBSEESA-M
InChi Code	InChI=1S/C36H60N5O7.ClH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-20-23-33(42)46-29-30(28-41(2,3)4)47-34(43)24-21-19-22-27-37-31-25-26-32(40(44)45)36-35(31)38-48-39-36;/h12-13,25-26,30,37H,5-11,14-24,27-29H2,1-4H3;1H/q+1;/p-1/b13-12-;
Chemical Name	trimethyl-[2-[6-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]hexanoyloxy]-3-[(Z)-octadec-9-enoyl]oxypropyl]azanium;chloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. DOTAP (and other cationic lipids): chemistry, biophysics, and transfection. Crit Rev Ther Drug Carrier Syst. 2004;21(4):257-317.

Solubility Data

Solubility (In Vitro)

DMSO : ~20 mg/mL (~28.16 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2 mg/mL (2.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4078 mL	7.0389 mL	14.0778 mL
	5 mM	0.2816 mL	1.4078 mL	2.8156 mL
	10 mM	0.1408 mL	0.7039 mL	1.4078 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.