Fluorescamine (Ro 20-7234) is a spirocyclic compound with no fluorescent properties. It can react with primary amines and form fluorescence, so it is used to detect amines and peptides.

Physicochemical Properties

Molecular Formula	C17H10O4
Molecular Weight	278.2589
Exact Mass	278.057
CAS #	38183-12-9
PubChem CID	37927
Appearance	White to light yellow solid powder
Density	1.4±0.1 g/cm3
Boiling Point	550.3±50.0 °C at 760 mmHg
Melting Point	153-157 °C(lit.)
Flash Point	248.5±30.2 °C
Vapour Pressure	0.0±1.5 mmHg at 25°C
Index of Refraction	1.687
LogP	2.95
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	21
Complexity	503
Defined Atom Stereocenter Count	0
InChi Key	ZFKJVJIDPQDDFY-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
Chemical Name	4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent. See also: Fluorescin (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO : ≥ 33 mg/mL (~118.59 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (8.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5938 mL	17.9688 mL	35.9376 mL
	5 mM	0.7188 mL	3.5938 mL	7.1875 mL
	10 mM	0.3594 mL	1.7969 mL	3.5938 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.