Fibrostat (Compound 5n) is a selective HDAC6 inhibitor that exerts anti-fibrotic function by inhibiting HDAC6 activity. The IC50 value for HDAC6 is 63 nM, and it shows good selectivity for HDAC1, HDAC3, HDAC5, HDAC8, HDAC10 and HDAC11. Fibrostat can significantly downregulate the expression of fibrosis markers (fibronectin and collagen 1) in fibroblasts. Fibrostat showed no toxicity in rat perfused heart and zebrafish larvae experiments. Fibrostat is expected to be used in the study of diseases related to fibrosis.

Physicochemical Properties

Molecular Formula	C29H32N4O3
Molecular Weight	484.59
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	HDAC6 63 nM (IC50)
References	[1]. Spirotetrahydroisoquinoline-Based Histone Deacetylase Inhibitors as New Antifibrotic Agents: Biological Evaluation in Human Fibroblasts from Bronchoalveolar Lavages of Idiopathic Pulmonary Fibrosis PatientsJ. ACS Pharmacology & Translational Science, 2024.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0636 mL	10.3180 mL	20.6360 mL
	5 mM	0.4127 mL	2.0636 mL	4.1272 mL
	10 mM	0.2064 mL	1.0318 mL	2.0636 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.