FeTCPP (Iron(III) meso-Tetra(4-carboxyphenyl)porphine chloride) is a metal porphyrin compound consisting of a central iron ion (Fe3+) coordinated with four 4-carboxyphenyl porphyrins (TCPP) form. FeTCPP could be utilized as a catalyst for catalytic, electrochemical, photochemical, and biomedical research. FeTCPP has efficient photocatalytic properties for p-nitrophenol under visible light. FeTCPP also has peroxidase-like activity and may be used in biomimetic catalysis research.

Physicochemical Properties

Molecular Formula	C48H28CLFEN4O8
Molecular Weight	880.06
Exact Mass	879.095
Elemental Analysis	C, 65.51; H, 3.21; Cl, 4.03; Fe, 6.35; N, 6.37; O, 14.54
CAS #	55266-17-6
Appearance	Brown to black solid powder
LogP	3.418
Synonyms	Fe(III) meso-Tetra(4-carboxyphenyl)porphine chloride; Fe (III) meso-Tetra(4-carboxyphenyl)porphine chloride;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Photocatalyst
ln Vitro	A photocatalyst of iron–porphyrin tetra-carboxylate (FeTCPP)-sensitized g-C3N4 nanosheet composites (FeTCPP@CNNS) based on g-C3N4 nanosheet (CNNS) and FeTCPP have been fabricated by in situ hydrothermal self-assembly. FeTCPP is uniformly introduced to the surface of CNNS. Only a small amount of FeTCPP is introduced, and the stacked lamellar structure is displayed in the composite. As compared with pure CNNS, the FeTCPP@CNNS composites exhibit significantly improved photocatalytic performance by the photodegradation of p-nitrophenol (4-NP). At the optimum content of FeTCPP to CNNS (3 wt%), the photodegradation activity of the FeTCPP@CNNS photocatalyst can reach 92.4% within 1 h. The degradation rate constant for the 3% FeTCPP@CNNS composite is 0.037 min−1 (4-NP), which is five times that of CNNS (0.0064 min−1). The results of recycling experiments show that 3% FeTCPP@CNNS photocatalyst has excellent photocatalytic stability. A possible photocatalytic reaction mechanism of FeTCPP@CNNS composite for photocatalytic degradation of 4-NP has been proposed. It is shown that superoxide radical anions played the major part in the degradation of 4-NP. The appropriate content of FeTCPP can enhance the charge transfer efficiency. The FeTCPP@CNNS composites can provide more active sites and accelerate the transport and separation efficiency of photogenerated carriers, thus further enhancing the photocatalytic performance.[1]
Enzyme Assay	Preparation of FeTCPP@CNNS[1] Typically, CNNS powder (1 g) was dispersed in 50 mL ethanol by ultrasonication. Then, a certain amount of FeTCPP was added to ethanol (10 mL), and the mixture was mixed into the above solution with magnetic stirring at 80 °C. Then, FeTCPP@CNNS composite material was obtained. The preparation process of FeTCPP@CNNS materials are shown in Figure 1. By this method, FeTCPP@CNNS composites with different FeTCPP contents (10 mg, 20 mg, 30 mg, and 40 mg) were prepared, which were denoted as X% FeTCPP@CNNS (X% = 1%, 2%, 3%, and 4%). Photocatalytic Assessment[1] The photocatalytic degradation of 4-NP was studied using xenon lamp (150 W) with filter as visible light source. Here, 50 mg photocatalyst was dispersed by magnetic stirring into 100 mL 4-NP aqueous solution (20 mg L−1). Firstly, the adsorption–desorption equilibrium was obtained by ultrasound for 0.5 h in the dark, then under visible light, and the reaction mixture was irradiated for 1 h. Then, 5 mL of the mixture was removed from the reactor, and at the same time interval, the concentration of 4-NP was determined by UV-Vis spectrophotometer. During the photocatalytic degradation of 4-NP, the effect of reactive oxygen species on the best-performing photocatalyst was tested for a scavengers, namely P-benzoquinone (BQ), isopropanol (IPA), and ethylenediamine tetraacetic acid disodium salt (EDTA-2Na). For this test, 1 mM scavenger was, respectively, added into 100 mL 4-NP solution (20 mg L−1), then was added 20 mg of catalyst. An additional procedure was carried out, which was the same as the process but without scavengers. Thus, all experiments were conducted under the identical conditions.
References	[1]. Fabrication of FeTCPP@ CNNS for Efficient Photocatalytic Performance of p-Nitrophenol under Visible Light. Catalysts, 2023, 13(4): 732.
Additional Infomation	In summary, the FeTCPP@CNNS photocatalysts with stacked lamellar structure have been successfully fabricated by an in situ hydrothermal self-assembly approach. The FeTCPP@CNNS composites exhibit higher photocatalytic efficiency and stability than CNNS by the photodegradation of 4-NP dyes. The photocatalytic degradation rate reached the maximum value of approximately 92.4% of 3% FeTCPP@CNNS. The degradation rate constant of the 3% FeTCPP@CNNS photocatalyst is 0.037 min−1 (4-NP), which is 5 times that of CNNS, indicating that proper FeTCPP introduced into CNNS can effectively improve transformation of photoexcitation electrons and holes. In addition, the results of the active species trapping experiments for the photodegradation of 4-NP show that ·O2− plays a major role in photocatalytic reactions. A possible photocatalytic reaction mechanism of FeTCPP@CNNS composite for photocatalytic degradation of 4-NP has been proposed. This work enables the application of CNNS-based photocatalysis under sunlight irradiation in wastewater treatment.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (28.41 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.84 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.84 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1363 mL	5.6814 mL	11.3629 mL
	5 mM	0.2273 mL	1.1363 mL	2.2726 mL
	10 mM	0.1136 mL	0.5681 mL	1.1363 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.