Fast Red ITR is a nonlinear optical material. Fast Red ITR may be utilized in alkaline phosphatase histochemistry.

Physicochemical Properties

Molecular Formula	C11H18N2O3S
Molecular Weight	258.3372
Exact Mass	258.103
CAS #	97-35-8
PubChem CID	66804
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	410.6±55.0 °C at 760 mmHg
Melting Point	102-105°C
Flash Point	202.1±31.5 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.547
LogP	1.65
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	17
Complexity	322
Defined Atom Stereocenter Count	0
SMILES	O=S(N(CC)CC)(C1C=C(N)C(OC)=CC=1)=O
InChi Key	WBGVVXSCGNGJFL-UHFFFAOYSA-N
InChi Code	InChI=1S/C11H18N2O3S/c1-4-13(5-2)17(14,15)9-6-7-11(16-3)10(12)8-9/h6-8H,4-5,12H2,1-3H3
Chemical Name	3-amino-N,N-diethyl-4-methoxybenzenesulfonamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Second-and third-harmonic generation of Nd: glass laser in fast red ITR organic crystal fiber. Japanese Journal of Applied Physics. 1998. [2]. A highly fluorescent simultaneous azo dye technique for demonstration of nonspecific alkaline phosphatase activity. J Histochem Cytochem. 1990 Mar;38(3):437-42.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~387.09 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.68 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8709 mL	19.3543 mL	38.7087 mL
	5 mM	0.7742 mL	3.8709 mL	7.7417 mL
	10 mM	0.3871 mL	1.9354 mL	3.8709 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.