FUBP1-IN-1 is a potent FUBP1 inhibitor that interferes with the binding of FUBP1 to the FUSE sequence of single-stranded target DNA, with IC50 of 11.0 μM.

Physicochemical Properties

Molecular Formula	C19H14F3N3O2S
Molecular Weight	405.393573284149
Exact Mass	405.075
CAS #	883003-62-1
PubChem CID	2823337
Appearance	Light yellow to yellow solid powder
LogP	4.2
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	4
Heavy Atom Count	28
Complexity	541
Defined Atom Stereocenter Count	0
InChi Key	CLFOYJSEPPNFQZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H14F3N3O2S/c1-26-14-6-5-11(8-15(14)27-2)12-9-17(19(20,21)22)25-18(23-12)10-13(24-25)16-4-3-7-28-16/h3-10H,1-2H3
Chemical Name	5-(3,4-dimethoxyphenyl)-2-thiophen-2-yl-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Pyrazolo[1,5a]pyrimidines as a new class of FUSE binding protein 1 (FUBP1) inhibitors. Bioorg Med Chem. 2016 Nov 15;24(22):5717-5729.

Solubility Data

Solubility (In Vitro)

DMSO : ~16.67 mg/mL (~41.12 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.79 mg/mL (4.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 17.9 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4668 mL	12.3338 mL	24.6676 mL
	5 mM	0.4934 mL	2.4668 mL	4.9335 mL
	10 mM	0.2467 mL	1.2334 mL	2.4668 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.