(±)-Evodiamine, a naturally occurring alkaloid, is an inhibitor (blocker/antagonist) of Top1. (±)-Evodiamine has anti-inflammatory, anti-obesity and anti-tumor effects. (±)-Evodiamine can inhibit the proliferation of various tumor cells by inducing apoptosis.

Physicochemical Properties

Molecular Formula	C19H17N3O
Molecular Weight	303.36
Exact Mass	303.137
CAS #	518-18-3
Related CAS #	Evodiamine;518-17-2
PubChem CID	151289
Appearance	Off-white to light brown solid powder
Density	1.4±0.1 g/cm3
Boiling Point	575.1±50.0 °C at 760 mmHg
Melting Point	272 °C
Flash Point	301.6±30.1 °C
Vapour Pressure	0.0±1.6 mmHg at 25°C
Index of Refraction	1.764
LogP	1.64
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	0
Heavy Atom Count	23
Complexity	495
Defined Atom Stereocenter Count	0
InChi Key	TXDUTHBFYKGSAH-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H17N3O/c1-21-16-9-5-3-7-14(16)19(23)22-11-10-13-12-6-2-4-8-15(12)20-17(13)18(21)22/h2-9,18,20H,10-11H2,1H3
Chemical Name	21-methyl-3,13,21-triazapentacyclo[11.8.0.02,10.04,9.015,20]henicosa-2(10),4,6,8,15,17,19-heptaen-14-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Top1
References	[1]. New tricks for an old natural product: discovery of highly potent evodiamine derivatives as novel antitumor agents by systemic structure-activity relationship analysis and biological evaluations. J Med Chem. 2012 Sep 13;55(17):7593-613.
Additional Infomation	LSM-6483 is a member of beta-carbolines.

Solubility Data

Solubility (In Vitro)

DMSO : 10 mg/mL (32.96 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (3.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2964 mL	16.4821 mL	32.9641 mL
	5 mM	0.6593 mL	3.2964 mL	6.5928 mL
	10 mM	0.3296 mL	1.6482 mL	3.2964 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.