Evocarpine is a quinolone alkaloid extracted from Evodiae fructus that can inhibit Ca2+ influx through voltage-dependent calcium channels. anti-bacterial effect.

Physicochemical Properties

Molecular Formula	C23H33NO
Molecular Weight	339.51
Exact Mass	339.256
CAS #	15266-38-3
PubChem CID	5317303
Appearance	White to off-white solid powder
Density	1.0±0.1 g/cm3
Boiling Point	456.2±45.0 °C at 760 mmHg
Melting Point	34-38℃
Flash Point	156.1±18.1 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.526
LogP	7.7
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	11
Heavy Atom Count	25
Complexity	451
Defined Atom Stereocenter Count	0
SMILES	CCCC/C=C\CCCCCCCC1=CC(=O)C2=CC=CC=C2N1C
InChi Key	HWFYWIVOYBPLQU-SREVYHEPSA-N
InChi Code	InChI=1S/C23H33NO/c1-3-4-5-6-7-8-9-10-11-12-13-16-20-19-23(25)21-17-14-15-18-22(21)24(20)2/h6-7,14-15,17-19H,3-5,8-13,16H2,1-2H3/b7-6-
Chemical Name	1-methyl-2-[(Z)-tridec-8-enyl]quinolin-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Evocarpine (1-100 μM) applied cumulatively suppresses the sustained induced contraction by 60 mM K+ in a concentration-dependent manner [1]. In promyelocytic leukemia HL-60 cells, evocarpine has been shown to cause apoptotic cell death in time- and dose-dependent ways[3].
References	[1]. The vasorelaxant effect of evocarpine in isolated aortic strips: mode of action. Eur J Pharmacol. 1988 Oct 11;155(1-2):139-43. [2]. Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine. J Appl Microbiol. 2015 Apr;118(4):864-72. [3]. Cyclic adenosine monophosphate inhibits quinolone alkaloid evocarpine-induced apoptosis via activation of protein kinase A in human leukaemic HL-60 cells. Pharmacol Toxicol. 2000 Jul;87(1):1-5.
Additional Infomation	Evocarpine is a member of quinolines. Evocarpine has been reported in Tetradium ruticarpum with data available.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (294.54 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.36 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.36 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9454 mL	14.7271 mL	29.4542 mL
	5 mM	0.5891 mL	2.9454 mL	5.8908 mL
	10 mM	0.2945 mL	1.4727 mL	2.9454 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.