Ethoxylated hydrogenated castor oil (PEG-40 hydrogenated castor oil) is a mixture of polyethylene glycol (PEG) and natural castor oil. Ethoxylated hydrogenated castor oil may be utilized to emulsify and solubilize oil-in-water (o/w) emulsions. Ethoxylated hydrogenated castor oil could be utilized as a cosolvent in animal experiments.

Physicochemical Properties

Molecular Formula	C21H23CLFNO2
Molecular Weight	375.864228487015
Exact Mass	375.14
CAS #	61788-85-0
Appearance	Colorless to light yellow liquid
Density	1.2±0.1 g/cm3
Boiling Point	529.0±50.0 °C at 760 mmHg
Flash Point	273.8±30.1 °C
Vapour Pressure	0.0±1.5 mmHg at 25°C
Index of Refraction	1.581
LogP	3.01
SMILES	C(CN1CCC(O)(C2C=CC(Cl)=CC=2)CC1)CC(=O)C1C=CC(F)=CC=1
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo

The mouse's left ear received 10 ul of 20% PEG-40 hydrogenated castor oil, while its right ear was used as a control. Six days went by during which the mouse rash developed, and the mice showed no symptoms of irritation [1]. For short-term toxicity, PEG-40 hydrogenated castor oil injections (tail vein injection; 300, 900, and 2700 mg/kg; 4 weeks) were utilized. At dosages of 300 and 900 mg/kg, PEG-40 hydrogenated castor oil did not exhibit any systemic toxicity. On the other hand, female body weight reduced little and male body weight decreased dramatically at the 2700 mg/kg dose [1]. Various oral active drugs were loaded into blank nanoemulsions for toxicity tests using PEG-40 hydrogenated castor oil as a surfactant [2]. Glyceryl monooleate (GMO) is emulsified as an oil into the aqueous phase using about 20% of PEG-40 hydrogenated castor oil. For 14 days in a row, 5000 mg/kg of blank nanoemulsion was given orally to Wechsler mice once daily. There were no negative effects from the nanoemulsion, and it had no influence on food consumption [2].

References

[1]. PEG-40 HYDROGENATED CASTOR OIL. [2]. The In Vitro-In Vivo Safety Confirmation of PEG-40 Hydrogenated Castor Oil as a Surfactant for Oral Nanoemulsion Formulation. Sci Pharm. 2017 Mar 31;85(2):18. doi: 10.3390/scipharm85020018. [3]. PEGylated Oils as Used in Cosmetics.

Solubility Data

Solubility (In Vitro)

H2O: ≥ 100 mg/mL

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6606 mL	13.3028 mL	26.6057 mL
	5 mM	0.5321 mL	2.6606 mL	5.3211 mL
	10 mM	0.2661 mL	1.3303 mL	2.6606 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.