EtS-DMAB (HClO-green) is a fluorescent probe that can selectively detect hypochlorous acid (HOCl) (λex=440 nm, λem=610 nm). EtS-DMAB may be utilized to image exogenous and endogenous HOCl in living cells.

Physicochemical Properties

Molecular Formula	C10H13N3OS
Molecular Weight	223.294720411301
Exact Mass	223.077
CAS #	2929446-76-2
PubChem CID	162642082
Appearance	Brown to reddish brown viscous liquid
LogP	2.2
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	15
Complexity	215
Defined Atom Stereocenter Count	0
SMILES	S(CC)C1=CC=C(C2C1=NON=2)N(C)C
InChi Key	AEMFWHVETFLRKQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H13N3OS/c1-4-15-8-6-5-7(13(2)3)9-10(8)12-14-11-9/h5-6H,4H2,1-3H3
Chemical Name	4-ethylsulfanyl-N,N-dimethyl-2,1,3-benzoxadiazol-7-amine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In an aqueous solution with a large Stokes shift (~170 nm), EtS-DMAB can selectively detect hypochlorous acid (HOCl) over other reactive oxygen species. As it turns out, the turn-on fluorescence is caused by the thioether being oxidized to the corresponding sulfoxide[1].
References	[1]. Highly selective fluorometric probes for detection of HClO in living cells. Sensors and Actuators B: Chemical. August 2018, 266 (1): 447-454.

Solubility Data

Solubility (In Vitro)

DMSO: 50 mg/mL (223.92 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.4785 mL	22.3924 mL	44.7848 mL
	5 mM	0.8957 mL	4.4785 mL	8.9570 mL
	10 mM	0.4478 mL	2.2392 mL	4.4785 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.