Endomorphin 2 TFA, a selective, high-affinity μ-opioid receptor agonist (activator), has high affinity for the kappa3 binding site with Ki between 20 and 30 nM.

Physicochemical Properties

Molecular Formula	C34H38F3N5O7
Molecular Weight	685.690039157867
Exact Mass	685.272
CAS #	1276124-00-5
Related CAS #	Endomorphin 2;141801-26-5
PubChem CID	137700452
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	12
Heavy Atom Count	49
Complexity	994
Defined Atom Stereocenter Count	4
SMILES	FC(C(=O)O)(F)F.O=C([C@@H]1CCCN1C([C@H](CC1C=CC(=CC=1)O)N)=O)N[C@H](C(N[C@H](C(N)=O)CC1C=CC=CC=1)=O)CC1C=CC=CC=1
InChi Key	ONJAQAAWBPIPNC-ZLYCMTQRSA-N
InChi Code	InChI=1S/C32H37N5O5.C2HF3O2/c33-25(18-23-13-15-24(38)16-14-23)32(42)37-17-7-12-28(37)31(41)36-27(20-22-10-5-2-6-11-22)30(40)35-26(29(34)39)19-21-8-3-1-4-9-21;3-2(4,5)1(6)7/h1-6,8-11,13-16,25-28,38H,7,12,17-20,33H2,(H2,34,39)(H,35,40)(H,36,41);(H,6,7)/t25-,26-,27-,28-;/m0./s1
Chemical Name	(2S)-1-[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]-N-[(2S)-1-[[(2S)-1-amino-1-oxo-3-phenylpropan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Pharmacological characterization of endomorphin-1 and endomorphin-2 in mouse brain. J Pharmacol Exp Ther. 1998 Aug;286(2):1007-13.

Solubility Data

Solubility (In Vitro)	H2O : ~62.5 mg/mL (~91.15 mM) DMSO : ~6 mg/mL (~8.75 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 33.33 mg/mL (48.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4584 mL	7.2919 mL	14.5838 mL
	5 mM	0.2917 mL	1.4584 mL	2.9168 mL
	10 mM	0.1458 mL	0.7292 mL	1.4584 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.