Eltoprazine HCl (formerly DU-28853; DU28853 hydrochloride) is a novel and potent serenic or antiaggressive agent which also acts as an agonist at the 5-HT1A and 5-HT1B receptors and as an antagonist at the 5-HT2C receptor. It might be used to treat ADHD, or attention deficit hyperactivity disorder.

Physicochemical Properties

Molecular Formula	C12H16N2O2
Molecular Weight	220.26764
Exact Mass	256.098
Elemental Analysis	C, 56.14; H, 6.67; Cl, 13.81; N, 10.91; O, 12.46
CAS #	98206-09-8
Related CAS #	Eltoprazine; 98224-03-4; Eltoprazine dihydrochloride; 143485-51-2
PubChem CID	3025067
Appearance	Solid powder
Density	1.18g/cm3
Boiling Point	384.3ºC at 760 mmHg
Flash Point	186.2ºC
Index of Refraction	1.562
LogP	2.063
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	17
Complexity	231
Defined Atom Stereocenter Count	0
SMILES	[H]Cl.N1(C2=C3OCCOC3=CC=C2)CCNCC1
InChi Key	JFSOSUNPIXJCIX-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H16N2O2.ClH/c1-2-10(14-6-4-13-5-7-14)12-11(3-1)15-8-9-16-12;/h1-3,13H,4-9H2;1H
Chemical Name	1-(2,3-dihydro-1,4-benzodioxin-5-yl)piperazine;hydrochloride
Synonyms	DU28853; DU 28853; DU-28853; Eltoprazine HCl
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	5-HT1A Receptor; 5-HT1B Receptor; 5-HT2C Receptor
ln Vivo	Long-term treatment with Mianserin (10 mg/kg, i.p.) or Eltoprazine (1 mg/kg, i.p.) and anxiety testing in the elevated plus maze test can be performed. Mianserin and Eltoprazine show opposite effects in the elevated plus maze: Mianserin produces anxiolytic-like effects, while Eltoprazine exhibits anxiolytic-like effects [2].
References	[1]. Eltoprazine, a drug which reduces aggressive behaviour, binds selectively to 5-HT1 receptor sites in the rat brain: an autoradiographic study. Eur J Pharmacol. 1990 Feb 20;177(1-2):55-66. [2]. Chronic mianserin or eltoprazine treatment in rats: effects on the elevated plus-maze test and on limbic 5-HT2C receptor levels. Eur J Pharmacol. 1994 Sep 1;262(1-2):125-31.

Solubility Data

Solubility (In Vitro)

H2O: ≥ 100 mg/mL (~389.5 mM) DMSO: ≥ 31 mg/mL (~120.8 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.74 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 100 mg/mL (389.51 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.5399 mL	22.6994 mL	45.3988 mL
	5 mM	0.9080 mL	4.5399 mL	9.0798 mL
	10 mM	0.4540 mL	2.2699 mL	4.5399 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.