Echistatin TFA is originally the smallest RGD active protein from the echistatin family and is a potent inhibitor of platelet aggregation. Echistatin is a potent inhibitor of bone resorption in vitro. Echistatin is a potent antagonist of αIIbβ3, αvβ3 and α5β1.

Physicochemical Properties

Molecular Formula	C219H342F3N71O76S9
Molecular Weight	5531.02
Related CAS #	Echistatin;154303-05-6
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets

αvβ3 α5β1 αIIbβ3

References

[1]. Inhibition of platelet adhesion to surfaces of extracorporeal circuits by disintegrins. RGD-containing peptides from viper venoms. Circulation. 1990 Jul;82(1):261-73. [2]. Echistatin is a potent inhibitor of bone resorption in culture. J Cell Biol. 1990 Oct;111(4):1713-23. [3]. Biochemical characterization of the binding of echistatin to integrin alphavbeta3 receptor. J Pharmacol Exp Ther. 1997 Nov;283(2):843-53. [4]. Structural requirements of echistatin for the recognition of alpha(v)beta(3) and alpha(5)beta(1) integrins. J Biol Chem. 1999 Dec 31;274(53):37809-14.

Solubility Data

Solubility (In Vitro)	H2O :~50 mg/mL (~9.04 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (9.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.1808 mL	0.9040 mL	1.8080 mL
	5 mM	0.0362 mL	0.1808 mL	0.3616 mL
	10 mM	0.0181 mL	0.0904 mL	0.1808 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.