Ecamsule disodium is a broad spectrum UVA sunscreen agent that may be utilized in sunscreen products. Ecamsule disodium reduces biological damage caused by solar radiation, such as the formation of pyrimidine dimers, the accumulation of p53 protein or the expression of collagenase 2.

Physicochemical Properties

Molecular Formula	C28H32NA2O8S2
Molecular Weight	606.66
Exact Mass	634.164
CAS #	90458-75-6
PubChem CID	77985954
Appearance	White to off-white solid powder
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	4
Heavy Atom Count	40
Complexity	1200
Defined Atom Stereocenter Count	0
InChi Key	ABZNEHKRFLKURR-UHFFFAOYSA-L
InChi Code	InChI=1S/C28H34O8S2.2Na/c1-25(2)21-9-11-27(25,15-37(31,32)33)23(29)19(21)13-17-5-7-18(8-6-17)14-20-22-10-12-28(24(20)30,26(22,3)4)16-38(34,35)36;;/h5-8,13-14,21-22H,9-12,15-16H2,1-4H3,(H,31,32,33)(H,34,35,36);;/q;2*+1/p-2
Chemical Name	disodium;[3-[[4-[[7,7-dimethyl-3-oxo-4-(sulfonatomethyl)-2-bicyclo[2.2.1]heptanylidene]methyl]phenyl]methylidene]-7,7-dimethyl-2-oxo-1-bicyclo[2.2.1]heptanyl]methanesulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

The greatest absorption of EcamsuLe, a broad spectrum UVA absorber, is measured at 344 nm [1]. The sensitivity of wild-type Fibs E6/E7 cells to EcamsuLe treatment (200–1600 μM) is higher [1]. EcamsuLe inhibits ROS production that is produced by UV and AAPH [1]. This impact is dose-dependent; in a UV environment, the largest reduction in ROS occurs at 1600 μM, the highest tested concentration. Both the basal level and the oxidative stress brought on by AAPH decreased by 16.9% and 10.8%, respectively, at the same concentration [1]. When ecamsuLe is applied at the maximum dose of 1600 μM, it boosts viability in AAPH-treated cells [1].

References

[1]. Contradictory effects of chemical filters in UV/ROS-stressed human keratinocyte and fibroblast cells. ALTEX. 2019;36(2):231-244. [2]. A new ecamsule-containing SPF 40 sunscreen cream for the prevention of polymorphous light eruption: a double-blind, randomized, controlled study in maximized outdoor conditions. Cutis. 2009 Feb;83(2):95-103.

Solubility Data

Solubility (In Vitro)

H2O: 50 mg/mL (82.42 mM) DMSO: 50 mg/mL (82.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6484 mL	8.2418 mL	16.4837 mL
	5 mM	0.3297 mL	1.6484 mL	3.2967 mL
	10 mM	0.1648 mL	0.8242 mL	1.6484 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.