HEPPS (EPPS) is a buffer solution with an effective pH range of 7.3 ~ 8.7. HEPPS reduces Aβ-aggregation-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally bioactive and can penetrate the BBB (blood-brain barrier).

Physicochemical Properties

Molecular Formula	C9H20N2O4S
Molecular Weight	252.3311
Exact Mass	252.114
CAS #	16052-06-5
PubChem CID	85255
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Melting Point	237-239 °C(lit.)
Index of Refraction	1.537
LogP	-2.46
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	6
Heavy Atom Count	16
Complexity	280
Defined Atom Stereocenter Count	0
InChi Key	OWXMKDGYPWMGEB-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
Chemical Name	3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. EPPS rescues hippocampus-dependent cognitive deficits in APP/PS1 mice by disaggregation of amyloid-β oligomers and plaques. Nat Commun. 2015 Dec 8;6:8997.
Additional Infomation	HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine.

Solubility Data

Solubility (In Vitro)	H2O : ~250 mg/mL (~990.77 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (396.31 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9631 mL	19.8153 mL	39.6306 mL
	5 mM	0.7926 mL	3.9631 mL	7.9261 mL
	10 mM	0.3963 mL	1.9815 mL	3.9631 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.