Physicochemical Properties
| Molecular Formula | C14H16N2O6 |
| Molecular Weight | 308.2866 |
| Exact Mass | 308.1 |
| CAS # | 55750-63-5 |
| PubChem CID | 5091655 |
| Appearance | White to off-white solid powder |
| Density | 1.4±0.1 g/cm3 |
| Boiling Point | 480.6±47.0 °C at 760 mmHg |
| Melting Point | 70-73 °C(lit.) |
| Flash Point | 244.5±29.3 °C |
| Vapour Pressure | 0.0±1.2 mmHg at 25°C |
| Index of Refraction | 1.577 |
| LogP | -0.81 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 8 |
| Heavy Atom Count | 22 |
| Complexity | 520 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | VLARLSIGSPVYHX-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C14H16N2O6/c17-10-5-6-11(18)15(10)9-3-1-2-4-14(21)22-16-12(19)7-8-13(16)20/h5-6H,1-4,7-9H2 |
| Chemical Name | (2,5-dioxopyrrolidin-1-yl) 6-(2,5-dioxopyrrol-1-yl)hexanoate |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | The peptide was connected to the 6-maleimidocaproate N-hydroxysuccinimide ester, which was subsequently attached to an adenovirus vector carrying a luciferase gene [1]. |
| References |
[1]. Studies on heterobifunctional cross-linking reagents, 6-maleimidohexanoic acid active esters. Chem Pharm Bull (Tokyo). 2007 Apr;55(4):685-7. [2]. Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier. Protein Pept Lett. 2010 Feb;17(2):164-7. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~230 mg/mL (~746.05 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 7.5 mg/mL (24.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 75.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 7.5 mg/mL (24.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 75.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 7.5 mg/mL (24.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 75.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2437 mL | 16.2185 mL | 32.4370 mL | |
| 5 mM | 0.6487 mL | 3.2437 mL | 6.4874 mL | |
| 10 mM | 0.3244 mL | 1.6218 mL | 3.2437 mL |