ELA-32(human) TFA is a potent, high-affinity apelin receptor blocker/inhibitor (IC50=0.27 nM; Kd=0.51 nM). ELA-32(human) TFA does not bind GPR15 and GPR25, but can activate the PI3K/AKT pathway and promote the self-renewal mechanism of hESCs through cell cycle and protein translation life processes. ELA-32(human) TF enhances the TGFβ pathway and directs hESCs toward the endodermal lineage. ELA-32(human) TF stimulates HUVEC cell angiogenesis.

Physicochemical Properties

Molecular Formula	C172H290F3N63O41S4
Molecular Weight	4081.84
Related CAS #	ELA-32(human);1680205-79-1
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~24.50 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (24.50 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.2450 mL	1.2249 mL	2.4499 mL
	5 mM	0.0490 mL	0.2450 mL	0.4900 mL
	10 mM	0.0245 mL	0.1225 mL	0.2450 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.