EGFR/VEGFR2-IN-4 (Compound 19) is an irreversible inhibitor of EGFR and VEGFR-2 with IC50 of 18.7 nM and 102.3 nM at 1 μM ATP.

Physicochemical Properties

Molecular Formula	C29H29N5O5
Molecular Weight	527.57
CAS #	870962-74-6
SMILES	CCOC1=C(NC(/C=C/CN(C)C)=O)C=C2C(N=CN=C2NC3=CC(C(OCC4=CC=CC=C4)=CC3=O)=O)=C1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	EGFR 18.7 nM (IC50); VEGFR2 102.3 nM (IC50)
References	[1]. Dual irreversible kinase inhibitors: quinazoline-based inhibitors incorporating two independent reactive centers with each targeting different cysteine residues in the kinase domains of EGFR and VEGFR-2. Bioorg Med Chem. 2007 Jun 1;15(11):3635-48.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8955 mL	9.4774 mL	18.9548 mL
	5 mM	0.3791 mL	1.8955 mL	3.7910 mL
	10 mM	0.1895 mL	0.9477 mL	1.8955 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.