EGFR-IN-144 (Compound 4B) exhibits inhibitory activity against EGFR (IC50=0.639 µg/mL) and tubulin polymerization (IC50=7.339 µg/mL). EGFR-IN-144 exhibits cytotoxicity in a variety of cancer cells with a GI50 of nanomolar levels. EGFR-IN-144 downregulates the expression of mTOR, TNF-α, and IL-6, arrests the cell cycle at the G1/S phase, and induces apoptosis.

Physicochemical Properties

Molecular Formula	C20H17CL2N3O3
Molecular Weight	418.27
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Development of 1, 2, 3-triazole hybrids as multi-faced anticancer agents co-targeting EGFR/mTOR pathway and tubulin depolymerizationJ. Bioorganic Chemistry, 2025: 108153.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3908 mL	11.9540 mL	23.9080 mL
	5 mM	0.4782 mL	2.3908 mL	4.7816 mL
	10 mM	0.2391 mL	1.1954 mL	2.3908 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.